Reproduction Technologies for Amphibians
Reproduction technologies (RTs) for amphibians have now reached the stage where
they con provide for the more cost efficient and secure management of Conservation Breeding
Programs (CBPs), the perpetuation of amphibian genetic variation, and for DNA related
conservation initiatives. This facility is mainly provided through the ability to cryopreserve
amphibian sperm and its use to produce offspring in CBS and by the
provision of genetic material for a range of conservation
purposes.
The use of reproduction technologies for amphibian conservation can be broadly
divided into; 1) the hormonal induction of sperm and oocytes (unfertilised eggs), 2) the short
term storage of sperm at refrigeration temperatures above freezing, 3) the long term storage of
sperm by cryopreservation, 4) artificial fertilisation either by simply mixing sperm and oocytes,
and 5) the injection of sperm into oocytes (ICSI - Intracytoplasmic sperm
injection.
Here we provide references and
analysis of research directly related to the
cryopreservation of amphibian sperm and research output over time. Sperm cryopreservation for
amphibians.
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